Journal: International Journal of Molecular Sciences

Article Title: α-Klotho Supplementation Mitigates Cumulative Exercise-Induced Fatigue via Coordinated NRF2-Mediated Antioxidant Defense and AKT/GS-Driven Hepatic Glycogen Supercompensation in Mice

doi: 10.3390/ijms27010412

Figure Lengend Snippet: α-Klotho treatment protects against exercise-induced fatigue. ( a ) Changes in mice bodyweight during 6-day exercise training ( n = 10 per group), ( b ) Mice bodyweight before sacrifice (day 7) ( n = 10 per group), ( c ) Grip strength of mice ( n = 10 per group), ( d ) Grip strength normalized by bodyweight, ( e ) Changes of time to exhaustion during exercise training ( n = 10 per group), changes of blood CK ( f ), BUN ( g ), and lactic acid ( h ) among groups ( n = 10 per group). Data represented as mean ± SD. * p < 0.05, ** p < 0.01; α-Klotho vs. Control, # p < 0.05; Saline vs. Control, & p < 0.05; α-Klotho vs. Saline, $ p < 0.05.

Article Snippet: After blocking with 5% non-fat milk, the membranes were incubated at 4 °C overnight with the following primary antibodies (all diluted 1:1000): α-Klotho (R&D Systems, Minneapolis, MN, USA, Cat# AF1819), NRF2 (Proteintech, Wuhan, Hubei, China, Cat # 16396-1-AP), p-NRF2 (Abways, Shanghai, China, Cat # CY6573), HO-1 (GenTex, Irvine, CA, USA, Cat # GTX13248), PGC-1α (Proteintech, Wuhan, Hubei, China, Cat # 66369-1-Ig), p-GS (Proteintech, Wuhan, Hubei, China, Cat # 28855-1-AP), GS (Proteintech, Wuhan, Hubei, China, Cat # 10566-1-AP), GLUT4 (Proteintech, Wuhan, Hubei, China, Cat # 66846-1-Ig), p-AKT (CST, Danvers, MA, USA, Cat # 4060S), t-AKT (CST, Danvers, MA, USA, Cat # 9272S), and GAPDH (CST, Danvers, MA, USA, Cat # 2118S).

Techniques: Control, Saline

Journal: International Journal of Molecular Sciences

Article Title: α-Klotho Supplementation Mitigates Cumulative Exercise-Induced Fatigue via Coordinated NRF2-Mediated Antioxidant Defense and AKT/GS-Driven Hepatic Glycogen Supercompensation in Mice

doi: 10.3390/ijms27010412

Figure Lengend Snippet: α-Klotho treatment attenuates oxidative stress in muscle. Changes of H 2 O 2 ( a ), MDA ( b ), SOD ( c ), T-GSH ( d ), GSSG ( e ), GSH ( f ), and GSH/GSSG ( g ) in muscle ( n = 10 per group). Data represented as mean ± SD. * p < 0.05, ** p < 0.01.

Article Snippet: After blocking with 5% non-fat milk, the membranes were incubated at 4 °C overnight with the following primary antibodies (all diluted 1:1000): α-Klotho (R&D Systems, Minneapolis, MN, USA, Cat# AF1819), NRF2 (Proteintech, Wuhan, Hubei, China, Cat # 16396-1-AP), p-NRF2 (Abways, Shanghai, China, Cat # CY6573), HO-1 (GenTex, Irvine, CA, USA, Cat # GTX13248), PGC-1α (Proteintech, Wuhan, Hubei, China, Cat # 66369-1-Ig), p-GS (Proteintech, Wuhan, Hubei, China, Cat # 28855-1-AP), GS (Proteintech, Wuhan, Hubei, China, Cat # 10566-1-AP), GLUT4 (Proteintech, Wuhan, Hubei, China, Cat # 66846-1-Ig), p-AKT (CST, Danvers, MA, USA, Cat # 4060S), t-AKT (CST, Danvers, MA, USA, Cat # 9272S), and GAPDH (CST, Danvers, MA, USA, Cat # 2118S).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: α-Klotho Supplementation Mitigates Cumulative Exercise-Induced Fatigue via Coordinated NRF2-Mediated Antioxidant Defense and AKT/GS-Driven Hepatic Glycogen Supercompensation in Mice

doi: 10.3390/ijms27010412

Figure Lengend Snippet: α-Klotho activates NRF2/HO-1 pathway in muscle. ( a ) Representative western blot band. Expression of α-klotho ( b ), p-NRF2/t-NRF2 ( c ), HO-1 ( d ), and PGC-1α ( e ) in muscle ( n = 6 per group). Data represented as mean ± SD. * p < 0.05, ** p < 0.01.

Article Snippet: After blocking with 5% non-fat milk, the membranes were incubated at 4 °C overnight with the following primary antibodies (all diluted 1:1000): α-Klotho (R&D Systems, Minneapolis, MN, USA, Cat# AF1819), NRF2 (Proteintech, Wuhan, Hubei, China, Cat # 16396-1-AP), p-NRF2 (Abways, Shanghai, China, Cat # CY6573), HO-1 (GenTex, Irvine, CA, USA, Cat # GTX13248), PGC-1α (Proteintech, Wuhan, Hubei, China, Cat # 66369-1-Ig), p-GS (Proteintech, Wuhan, Hubei, China, Cat # 28855-1-AP), GS (Proteintech, Wuhan, Hubei, China, Cat # 10566-1-AP), GLUT4 (Proteintech, Wuhan, Hubei, China, Cat # 66846-1-Ig), p-AKT (CST, Danvers, MA, USA, Cat # 4060S), t-AKT (CST, Danvers, MA, USA, Cat # 9272S), and GAPDH (CST, Danvers, MA, USA, Cat # 2118S).

Techniques: Western Blot, Expressing

Journal: International Journal of Molecular Sciences

Article Title: α-Klotho Supplementation Mitigates Cumulative Exercise-Induced Fatigue via Coordinated NRF2-Mediated Antioxidant Defense and AKT/GS-Driven Hepatic Glycogen Supercompensation in Mice

doi: 10.3390/ijms27010412

Figure Lengend Snippet: α-Klotho increases liver glycogen content. Glycogen content in muscle ( a ) and the liver ( b ) ( n = 10 per group). Data represented as mean ± SD. ** p < 0.01.

Article Snippet: After blocking with 5% non-fat milk, the membranes were incubated at 4 °C overnight with the following primary antibodies (all diluted 1:1000): α-Klotho (R&D Systems, Minneapolis, MN, USA, Cat# AF1819), NRF2 (Proteintech, Wuhan, Hubei, China, Cat # 16396-1-AP), p-NRF2 (Abways, Shanghai, China, Cat # CY6573), HO-1 (GenTex, Irvine, CA, USA, Cat # GTX13248), PGC-1α (Proteintech, Wuhan, Hubei, China, Cat # 66369-1-Ig), p-GS (Proteintech, Wuhan, Hubei, China, Cat # 28855-1-AP), GS (Proteintech, Wuhan, Hubei, China, Cat # 10566-1-AP), GLUT4 (Proteintech, Wuhan, Hubei, China, Cat # 66846-1-Ig), p-AKT (CST, Danvers, MA, USA, Cat # 4060S), t-AKT (CST, Danvers, MA, USA, Cat # 9272S), and GAPDH (CST, Danvers, MA, USA, Cat # 2118S).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: α-Klotho Supplementation Mitigates Cumulative Exercise-Induced Fatigue via Coordinated NRF2-Mediated Antioxidant Defense and AKT/GS-Driven Hepatic Glycogen Supercompensation in Mice

doi: 10.3390/ijms27010412

Figure Lengend Snippet: α-Klotho activates the AKT signaling pathway in the liver. ( a ) Representative western blot band. Expression of α-klotho ( b ), p-GS/t-GS ( c ), t-GS ( d ), p-AKT/t-AKT ( e ), GLUT4 ( f ), and PGC-1α ( g ) in the liver ( n = 6 per group). Data represented as mean ± SD. * p < 0.05, ** p < 0.01.

Article Snippet: After blocking with 5% non-fat milk, the membranes were incubated at 4 °C overnight with the following primary antibodies (all diluted 1:1000): α-Klotho (R&D Systems, Minneapolis, MN, USA, Cat# AF1819), NRF2 (Proteintech, Wuhan, Hubei, China, Cat # 16396-1-AP), p-NRF2 (Abways, Shanghai, China, Cat # CY6573), HO-1 (GenTex, Irvine, CA, USA, Cat # GTX13248), PGC-1α (Proteintech, Wuhan, Hubei, China, Cat # 66369-1-Ig), p-GS (Proteintech, Wuhan, Hubei, China, Cat # 28855-1-AP), GS (Proteintech, Wuhan, Hubei, China, Cat # 10566-1-AP), GLUT4 (Proteintech, Wuhan, Hubei, China, Cat # 66846-1-Ig), p-AKT (CST, Danvers, MA, USA, Cat # 4060S), t-AKT (CST, Danvers, MA, USA, Cat # 9272S), and GAPDH (CST, Danvers, MA, USA, Cat # 2118S).

Techniques: Western Blot, Expressing

Journal: bioRxiv

Article Title: sKL/mKL Transcript Ratio and Protein Localization Define a Species- and Region-Specific Klotho Signature in the CNS and AD Progression

doi: 10.1101/2025.05.13.653838

Figure Lengend Snippet: Representative images of double immunolabelling sections from human hippocampus and amygdala for total klotho and s-KL (A) ; or for s-KL and GFAP (B) . Negative controls were done by incubating sections without the primary antibodies or by incubating with the pre-adsorbed antibody. White arrows in (B) point to the few neurons expressing s-KL, while yellow labelling indicates s-KL positive astrocytes. Bar scale size is 10μm. Quantification of total soluble klotho (KL1) and s-KL in CSF (C) and homogenized brain tissue (HC: hippocampus, EC: entorhinal cortex) (D) by ELISA.

Article Snippet: The primary antibody, mouse monoclonal klotho antibody F-5 (Santa Cruz Biotechnology, ref sc-515939), diluted 1/100 in blocking buffer, was applied overnight at 4°C.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay